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Isolation, Identification, Purification and Characterization of Antibiotic Producing Bacteria from Different Soil Samples

Streptococcus and Micrococcus luteus are the best source of antibiotic. In the present study 4 culture (Bacillus cereus(S6), Pseudomonas fluoresens(S1), Streptococcus(S2), Micrococcus luteus(S3) were isolated from 6 soil sample collected from Lucknow, Kanpur and Unnao. Isolation of microbes was done by serial dilution method. Primary screening of the culture was done by streaking. Gram’s staining was done to check the culture was pure or not. Identification was done by Bergey’s manual, further media optimization was done. Production media was prepared for these 4 culture in which the best carbon sources was Sucrose, Glucose, Dextrose, Sucrose best nitrogen sources were Yeast extract, the best temperature was 370C and best pH was 11,7,7,7(Micrococcus luteus, Streptococcus, Pseudomonas fluorescens, Bacillus cereus) respectively. Further extraction of seconda ry metabolite was done by using one solvent :(Chloroform) for extracellular component and (Methanol) for the intracellular component. Further purification of the antimicrobial compound using TLC (thin layer chromatography) and a spectrophotometer was performed resulting Quercetin compound.

Published by: Rajnish Vishwakarma

Author: Rajnish Vishwakarma

Paper ID: V3I5-1201

Paper Status: published

Published: September 26, 2017

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Research Paper

Mid-Point Algorithm Based Sink Movement to Improve Lifetime of the Wireless Sensor Networks

The wireless sensor networks with the mobile sink are more effective in providing better network performance as compared to the ones with the static sinks. This paper proposes a scheme cluster based WSN with the tour planning of the mobile sink. Since the deployment of the nodes in the WSN is purely random, this leads to some nodes in the network not connected to any cluster (when the clustering is done). The proposed scheme takes into consideration cluster heads and unconnected nodes to plan the trajectory of the sink movement. The scheme was implemented in NS2.35 and compared with the existing approach based on the remaining energy, routing overhead and packet delivery ratio. The scheme has outperformed the existing scheme.

Published by: Ankush Bhatt, Geetika Gandhi

Author: Ankush Bhatt

Paper ID: V3I5-1195

Paper Status: published

Published: September 26, 2017

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Research Paper

Various Mobile Sink Approaches for data aggregation in WSN: A Survey

A WSN is made of one or numerous distant sinks and numerous minute, low-power sensors, apiece equipped with actuators, sensing devices, and wireless transmitters and receivers. Sensors are generally power-driven by smaller batteries. The sink is normally static and located outside the network. This results in the increase of the communication distance between the far away cluster heads from the sink. These spent higher energies than those located close to sink. In order to balance the energy, this paper displays various clustering approaches that makes the use of the mobile sink to collect data from the cluster heads.

Published by: Ankush Bhatt, Geetika Gandhi

Author: Ankush Bhatt

Paper ID: V3I5-1194

Paper Status: published

Published: September 26, 2017

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Research Paper

CFD Based Quantification of Aerodynamic Performance of an Automotive With Roof Dimples

The impact of aerodynamics on vehicle performance has gained greater importance in the modern days. The effecst of drag and lift forces on automotives have been well accounted for and engineers are relentlessly working on minimising the negative implications of these aerodynamic forces. The dimples on golf balls have been proven to reduce drag and are given credit for a golf ball’s longer flight. The use of dimples to reduce drag has often inspired many aerodynamists and this research aims to quantify the drag and lift forces on an automotive with dimples on its roof. A wide range of dimple ratios, arrangements and velocities have been taken into consideration and successful reduction in drag and lift coefficients has been reported.

Published by: Akshay Ashok Kumar

Author: Akshay Ashok Kumar

Paper ID: V3I5-1198

Paper Status: published

Published: September 26, 2017

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Research Paper

Influence of Age and Gender on Interpupilary Distance and Comparison of Pd Ruler and Auto Refractrometer Values of Interpupilary Distance

Information about the normal values of interpupilary distance (IPD) for particular age group, gender and ethnic background has important clinical implications. Normative values of IPD could be useful in the diagnosis of certain syndromes and also in surgery after facial trauma. Our main aim to carrying out this study was to find out the effect of gender and age on interpupilary distance in total study sample of 30 subjects within the age limit of 15 to 60 years and IPD measurements were compared taken with two different instruments i.e. PD ruler and auto refractrometer. Data was collected using descriptive cross sectional study at Ophthalmology Department at Medina Teaching Hospital, Faisalabad. Proper room for examination with all instruments that were used in study was chosen after history taking. With torch light we examined that the pupil is round, regular and reactive. Then IPD values were measured using a millimeter PD ruler and auto refractrometer. Our study results show that gender has an effect on IPD measurements and also that males have 3mm wider papillary distance than females. Our study subjects were divided into 3 age groups and IPD in younger age (15-30 years) group keep on increasing, after wards it remains constant. And another objective of our study was to compare IPD values with different instruments i.e. PD ruler and auto refractrometer used and results are significant. It is suggested that IPD should be an integral part of eye examination to avoid any future intolerance of spectacles and asthenopic symptoms.

Published by: Zakia Batool, Dr. Waqas Mahmood, Umar Waqas Khan

Author: Zakia Batool

Paper ID: V3I5-1173

Paper Status: published

Published: September 26, 2017

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Research Paper

Use of Demolished Waste in Partial Replacement Of Course Aggregate With Concrete

There is Associate in Nursing outsized amount of demolished waste generated annually in Republic of Asian country and various developing countries. Since very little bit of this waste is recycled or reused. So, disposing this waste is also a really major drawback as a results of it desires Associate in Nursing outsized amount of house. For the study seven, fourteen and twenty eight days compressive strengths were recorded.

Published by: Akash Chandra, Sri Hari Reddy, Gokulnath.V

Author: Akash Chandra

Paper ID: V3I5-1193

Paper Status: published

Published: September 26, 2017

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