This paper is published in Volume-5, Issue-5, 2019
Area
Genetic Engineering
Author
Ketaki Ghatole
Co-authors
Shruthi Mohan, Hashvitha R., Meghana K. J., Suresh H. Antre
Org/Univ
Ramaiah Institute of Technology, Bangalore, Karnataka, India
Pub. Date
30 October, 2019
Paper ID
V5I5-1323
Publisher
Keywords
Oryza sativa, RAPD markers, PCR, Polymorphism, Genetic diversity

Citationsacebook

IEEE
Ketaki Ghatole, Shruthi Mohan, Hashvitha R., Meghana K. J., Suresh H. Antre. Molecular marker analysis of Oryza sativa using RAPD markers, International Journal of Advance Research, Ideas and Innovations in Technology, www.IJARIIT.com.

APA
Ketaki Ghatole, Shruthi Mohan, Hashvitha R., Meghana K. J., Suresh H. Antre (2019). Molecular marker analysis of Oryza sativa using RAPD markers. International Journal of Advance Research, Ideas and Innovations in Technology, 5(5) www.IJARIIT.com.

MLA
Ketaki Ghatole, Shruthi Mohan, Hashvitha R., Meghana K. J., Suresh H. Antre. "Molecular marker analysis of Oryza sativa using RAPD markers." International Journal of Advance Research, Ideas and Innovations in Technology 5.5 (2019). www.IJARIIT.com.

Abstract

Rice is one of the most important crops that provides food for more than half of the world’s population and hence their cultivation is expanding drastically due to the increasing demand. There are different varieties of rice distinguished based on their physiological characteristics and geographical area. Identification of these genotypes are important during the genetic breeding programs. Molecular markers play a crucial role in studying genetic variation among different species. The analysis of this diversity among six varieties of rice: Sahabagi Dhan, IR-64, MGD-101, BPT-5204, Azucena, Doddiga was performed by extracting DNA and using 8 RAPD markers on them. The random DNA sequences amplified by PCR are compared and analysed by the process of electrophoresis. The binary of the gel images is used for analysis of genetic variation between the cultivars. It is observed that the number of polymorphic fragments for each primer varied from from 3 to 7 with an average of 4. The average percentage polymorphism was calculated as 77.573%. The values of similarity co-efficient ranged from 0.41 to 0.85. The values of similarity co-efficient ranged from 0.41 between MGD-101 and IR-64 to 0.85 between BPT-5204 and Azucena and an average of 0.71. Cluster analysis showed that IR-64 and MGD-101 are bifolious and Azucena is a simplifolious indicating that it is a wild variety and is substantially different from all others. Major Allele Frequency ranges from 0.61 to 0.90 with a mean of 0.79, Gene Diversity values range from 0.17 to 0.46 with a mean of 0.29 and PIC values range from 0.14 to 0.36 with a mean of 0.23. The information obtained in this study helps to understand the genetic diversity. It can be used to emphasize conservation and propagation of selected natural varieties and to select diverse parents to broaden the germplasm of rice.
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