This paper is published in Volume-4, Issue-4, 2018
Area
Agriculture
Author
Gorad D. S.
Co-authors
Shaikh S. A., Kallalbandi B. M., Mali A. S.
Org/Univ
Shriram College Of Agriculture, Paniv, Maharashtra, India
Pub. Date
09 July, 2018
Paper ID
V4I4-1191
Publisher
Keywords
Micro propagation, In vitro, Pomegranate, Bhagwa

Citationsacebook

IEEE
Gorad D. S., Shaikh S. A., Kallalbandi B. M., Mali A. S.. In vitro propagation of pomegranate, International Journal of Advance Research, Ideas and Innovations in Technology, www.IJARIIT.com.

APA
Gorad D. S., Shaikh S. A., Kallalbandi B. M., Mali A. S. (2018). In vitro propagation of pomegranate. International Journal of Advance Research, Ideas and Innovations in Technology, 4(4) www.IJARIIT.com.

MLA
Gorad D. S., Shaikh S. A., Kallalbandi B. M., Mali A. S.. "In vitro propagation of pomegranate." International Journal of Advance Research, Ideas and Innovations in Technology 4.4 (2018). www.IJARIIT.com.

Abstract

An investigation on “Micropropagation of pomegranate (PunicagranatumL.) Var. Bhagwan” In the present investigation total five sub-experiments was carried out. In which CRD was applied for a laboratory experiment and RBD for field experiment to find out best explants, surface sterilants, shooting media, rooting media, and hardening mixtures. Regarding the suitability of explants, shoot tip was best for culture establishment. The study on surface sterilization revealed that explants treated with 0.1 percent mercuric chloride for 10 minutes showed the maximum survival percentage (90.58%) and less contamination percentage (9.42) in shoot tip explants. Shoot differentiation study showed that maximum number of shots per shoot tip explant (1.73) was recorded in treatment containing MS + BAP 2.0 mg/l, maximum shoot length (2.80 cm) was observed on medium containing MS + BAP 2.5 mg/l and maximum number of leaves (7.73) was recorded on medium containing MS +BAP 2.5mg/among the auxins used in the rooting experiment the maximum number of roots(9.66) per shoot tip explant was recorded in treatment containing ½ MS + NAA 6 mg/l. Maximum root length (3.20 cm) was found on medium containing ½ MS + IBA mg/l. While hardening in different hardening media, it was observed that maximum (71.87%) survival of plantlets from shoot tip explants was found on medium containing soil + sand (1:1v/v).
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