Introduction: Many of Hindu devotees undergo an annual pilgrimage to the Amarnath cave. During ascent for Amarnath Yatra in the mountains they experience multiple environmental stressors, but the stress unique to high altitudes is the hypoxia. Oxygen saturation is defined as the fraction of oxygen-saturated hemoglobin relative to total hemoglobin in the blood. The present study was designed with the aim to assess oxygen saturation of Amarnath Yatris at the holy Amarnath cave. Material and Methods: A total of 75 subjects of both male and female of different age groups were taken. An informed consent was taken from each subject before the cross sectional study. Peripheral Oxygen Saturation (SpO2) was measured with pulse oximetry process. Result: There was a significant decrease in Peripheral Oxygen Saturation (SpO2) in females was more than male Yatris with a mean of 67.7% as compared to 76%. Conclusion: In the study, there was a significant decrease in Peripheral Oxygen Saturation (SpO2) in all the subjects at the height of the Amarnath cave. Keywords: Peripheral oxygen saturation (SpO2), Amarnath Yatris, pulse oximeter, hypoxia, high altitude.