In response to pandemics and microbial resistance, novel heterocyclic compound spotent biological activity are needed. A series of (E)-2-(2-((5-(1H-benzo[d][1,2,3]triazol-1-yl)-3- methyl-1-phenyl-1 H-pyrazol-4-methylene(hydrazinyl)-4-(aryl) thiazole derivatives were synthesized via a three- component reaction involving pyrazole-4-carbaldehyde, thio semicarbazide, and substituted phenacyl bromides for antibacterial and antifungal study. Recent health research focuses on multifunctional compounds that interact with multiple biological targets, streamlining multidrug therapies and enhancing patient adherence. This study aimed to develop novel multifunctional chemical entities incorporating a benzothiazole nucleus, a structure widely recognized for its diverse biological activities. Benzothiazole has gained attention due to its role as a scaffold in various multifunctional drugs, making it a promising candidate for innovative therapeutic applications that improve treatment efficacy and simplify pharmaceutical regimens. To combat the growing threat of multi-resistant bacteria, scientists synthesized four benzotriazole and three benzimidazole derivatives using two distinct methods, recognizing the vital role of heterocyclic compounds in medicinal chemistry. These newly developed compounds were then docked with two protein targets, DNA gyrase (PDB ID: 2XCT and 3ILW), to evaluate their binding potential. The effectiveness of these derivatives was compared with standard antibacterial drugs, sparfloxacin and ciprofloxacin. This study aims to identify promising candidates for overcoming bacterial resistance, providing valuable insights into new drug development strategies targeting resistant bacterial strains through advanced molecular docking techniques. Antimicrobial resistance (AMR) is a global health challenge, leading to higher mortality, morbidity, and treatment costs. The World Health Organization (WHO) reported in 2019 that only six out of 32 antibiotics in clinical trials featured innovative novel moieties, while the rest were based on existing compounds. This highlights the urgent need for new antibiotic development to combat resistance. Among promising candidates, benzothiazole derivatives stand out due to their broad spectrum of biological activities and significant medicinal applications. Their potential in drug discovery has gained attention for addressing resistance issues, reinforcing the necessity of developing novel compounds. Advancing research in benzothiazole derivatives may pave the way for effective antimicrobial agents to tackle evolving resistance problems and improve global healthcare outcomes.