One of the most prevalent diseases in the world is cancer. In order to stop cancer from growing, developing, and spreading, cancer treatments still need to be carefully planned and targeted. Radiation, cellular stress, and cytotoxic medications are some of the triggers that activate the mitochondrial intrinsic apoptotic pathway. Native to the Mediterranean region, Vinca roseus is a blooming perennial plant that is primarily found in the northern hemisphere. They are native to tropical regions in South Asia. There are numerous uses for the Madagascar plant, Catharanthus roseus L. The 3D crystallographic structure of the Anti-cancer receptor (PDB ID-2KCE & 2HBS ) was obtained from the Protein Data Bank and utilized as a protein target for in-silico experiments. Molecular docking was performed using Auto Dock 4 and Auto Dock Vina. A blind docking approach was employed to encompass all possible ligand binding sites. The binding free energy (kcal/mol) was utilized to calculate the binding affinity. This study reveals that Catharanthus roseus, a polyphenolic compound, may possess antioxidant, antibacterial, antifungal,&anticancer activity against the Anti-cancer receptor responsible for cancer disease, as predicted in silico. Molecular docking data suggest that Vincristine(-7.1& -9.5 Kcal/mol) and Vindesine (-9.1 & -10.5 Kcal/mol) have greater activity than Vincristine. They have good Anticancer properties. & The best protein was found to be 2HBS for Anti-cancer activity.