Gallic acid is a bioactive phenolic compound widely recognized for its antioxidant and pharmacological properties. The present study qualitatively investigates the presence of gallic acid in root samples of Rauvolfia serpentina (Sarpagandha) collected from four distinct agro-climatic zones of India using High Performance Thin Layer Chromatography (HPTLC). Methanolic extracts of root samples were analyzed alongside a gallic acid standard employing a standardized solvent system. The chromatographic profiles of all samples exhibited peaks corresponding to the retention factor (Rf) range of the reference standard, confirming the presence of gallic acid across all evaluated samples. This study supports the phytochemical consistency of Rauvolfia serpentina roots and contributes to quality control and standardization efforts of this important medicinal plant.