Biosurfactants are bioactive surface molecules produced by microorganisms, gaining notoriety for their environmentally friendly and biodegradable characteristics. This research emphasizes the extraction, production, and analysis of biosurfactants from hydrocarbon-polluted soils collected from a garage and truck terminal in the Yeshwanthpur industrial region. The samples were enriched using Mineral Salt Medium (MSM), and bacterial strains were isolated through serial dilution and pour plate methods. The identification of biosurfactant-producing bacteria was performed utilizing drop collapse, oil displacement, and emulsification assays. Among the isolates, isolate 2 exhibited the most promising results and was chosen for further research. Gram staining, endospore staining, and biochemical tests revealed the organism to be Bacillus cereus. Optimization of biosurfactant production was achieved by adjusting pH, temperature, incubation duration, inoculum volume, and nutrient sources. The maximum biosurfactant yield was attained with 250 µl of inoculum and with optimum physical parameters of pH 6 and temperature 35°C at a 24-hour incubation period, with glucose and peptone as carbon and nitrogen sources, respectively. The biosurfactants were extracted through acid precipitation followed by solvent extraction using chloroform and methanol. The characterization of the crude biosurfactant was performed. The antimicrobial properties against selected bacterial and fungal strains were assessed using the agar diffusion method, and bioremediation potential was evaluated. Distinct zones of inhibition confirmed the antimicrobial efficacy of the biosurfactant. These results imply that Bacillus cereus isolated from garage soil contains effective biosurfactant-producing potential and can be used in environmental bioremediation and antimicrobial property, offering a sustainable substitute for synthetic surfactants.