This paper is published in Volume-5, Issue-4, 2019
Area
Bioengineering
Author
Sanyam Modi
Co-authors
Chintan Somani
Org/Univ
Indian Institute of Technology, New Delhi, Delhi, India
Pub. Date
06 August, 2019
Paper ID
V5I4-1303
Publisher
Keywords
Silica Coated Magnetic Nanoparticles

Citationsacebook

IEEE
Sanyam Modi, Chintan Somani. Development of DNA isolation and purification method with automation by using Silica coated Magnetic Nanoparticles, International Journal of Advance Research, Ideas and Innovations in Technology, www.IJARIIT.com.

APA
Sanyam Modi, Chintan Somani (2019). Development of DNA isolation and purification method with automation by using Silica coated Magnetic Nanoparticles. International Journal of Advance Research, Ideas and Innovations in Technology, 5(4) www.IJARIIT.com.

MLA
Sanyam Modi, Chintan Somani. "Development of DNA isolation and purification method with automation by using Silica coated Magnetic Nanoparticles." International Journal of Advance Research, Ideas and Innovations in Technology 5.4 (2019). www.IJARIIT.com.

Abstract

Development of DNA isolation and purification method with automation by using Silica coated Magnetic Nanoparticles. To develop a protocol to extract and purify the DNA, with automation using silica-coated magnetic particles, of two gram-negative bacteria E. coli DH5α for small volume culture (1ml) and large volume culture (5ml). Cell Lysis: Here all the analysis has been done on E. coli DH5α, gram negative bacteria. Washing: The second step comes in purifying the nucleic acids is the washing. Other than proteins, there are many kinds of impurities present with nucleic acids and these impurities need to be washed. Elution: The final step is the elution step. In this step, finally the DNA is extracted from the silica coated MNPs and hence purified.
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