Aswini Hiran Strong Pain Oil is a topical Ayurvedic pain-relieving preparation used for knee pain, joint pain, muscular pain, shoulder pain, and backache. The product claims a rapid onset of relief within minutes of application and significant improvement within 14 days of regular use. A prospective, open-label, Phase 4 clinical study was conducted on adult subjects (n = 30) experiencing musculoskeletal pain. Pain severity was measured using a Visual Analogue Scale (VAS: 0–4) at baseline (Day 0) and after 14 days of regular application (Day 14). The study demonstrated significant improvement across all pain categories, with p < 0.001 for every parameter evaluated through a two-tailed paired t-test analysis. Mean pain reduction at Day 14 was 81.25% for knee pain, 78.13% for back pain, 89.29% for shoulder pain, and 78.57% for calf/muscle pain. Onset-of-relief assessments showed 80% of participants experienced noticeable relief within 10 minutes, with 10% reporting relief as early as 3 minutes. No adverse reactions or tolerability issues were reported. These findings substantiate the claims of Aswini Hiran Strong Pain Oil and confirm its effectiveness as a topical remedy for musculoskeletal pain.