In the present project, the synthesis of substituted benzopyran derivatives have been reported as one-pot reaction by reaction of 2,2 Dimethyl-2,7b dihydro-1 aH- oxireno[2,3-c] chromen-5-yl acetate with ethanol and 4- methyl benzylamine. The synthesized compounds in this work were screened in vitro for their antimicrobial activity against some strains of bacteria. The antibacterial activities of synthesized compounds were compared with the antibacterial activity of the standard antibiotics Ciprofloxacin. The tested compounds revealed antibacterial properties. This review is summarized to know about the different pharmacological activities of Benzopyran nucleus with the extended knowledge about its antimicrobial activity.