Antibiotic resistance presents a global health crisis, where bacteria evolve to withstand antimicrobial treatments, increasing mortality rates. This escalating threat, even though a natural evolutionary process, has been significantly accelerated by the pervasive misuse and overuse of antibiotics in both human and veterinary medicine. The staggering statistics, including millions of infections and thousands of deaths annually in the United States alone, underscore the urgent need for innovative solutions. Among the most promising advancements are biosensors, analytical devices comprising a biorecognition element and a transducer. These instruments offer rapid, sensitive, and precise detection of pathogens and antibiotic residues. Various biosensors are being developed and deployed to identify resistant microbial strains. Biosensors are a pivotal tool in mitigating the deadly impact of antimicrobial resistance and safeguarding public health.