The Azoxystrobin and Difenoconazol molecules are being used alone and combinations system as a fungicide in the cultivation industry. Such an important combination molecule has to be analyzed to estimate the active content exactly with less time and cost of analysis. A simple analytical method developed to analyze these two molecules within 15 minutes of analysis time. The separation was achieved through the Shimadzu BDS C18 (250 x 4.6 mm, 5μm) column with the flow rate as 1.0 ml/min with the detection at 230 nm. These method parameters were loaded in the Shimadzu HPLC (model: LC-2030) with acetonitrile and water (HPLC grade) at 60:40 ratio as the mobile phase. Validated shimadzu Liquid Chromatography solution software was used for all the calculations in this analytical method validation analysis. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the identification and quantifications of these molecules interims of validation parameters viz., separation, system suitability, System Precision and linearity in a simple HPLC analysis.