The interest of using alternative fuels in diesel engines has been accelerated exponentially due to a foreseen scarcity in world petroleum reserves, increase in the prices of the conventional fossil fuels and restrictions on exhaust emissions such as greenhouse gases from internal combustion (IC) engines initiated by environmental concerns. Pyrolysis oil is used to be fueled with diesel. The usage of oxygenated fuel will reduce emission level. Adding Pyrolysis oil to petroleum products allows the fuel to combust properly due to the presence of oxygen, which increases the combustion efficiency and reduces air pollution. The higher activation energy of alcohols leads to better resistance to engine knocking that allows higher compression ratios and greater engine thermal efficiencies. In this study, by varying the engine load and Pyrolysis oil percentage (10% & 20%) with Diesel in DI Diesel engine the influence of Pyrolysis oil /diesel fuel blends has to be analyzed while comparing the performance and emissions characteristics with diesel fuel. The properties of the mixtures were estimated and the exhaust gas components such as hydrocarbons, CO, CO2, and NOx emissions have also been measured with the gas analyzer and have been compared with base fuel.