This paper is published in Volume-6, Issue-4, 2020
Area
Haematology
Author
Dr. Muthu Venkat T.
Org/Univ
Independent Researcher, India
Pub. Date
15 July, 2020
Paper ID
V6I4-1193
Publisher
Keywords
βTT, electrophoresis, RBC Indices.

Citationsacebook

IEEE
Dr. Muthu Venkat T.. Clinico haematological profile in beta Thalassemia trait, International Journal of Advance Research, Ideas and Innovations in Technology, www.IJARIIT.com.

APA
Dr. Muthu Venkat T. (2020). Clinico haematological profile in beta Thalassemia trait. International Journal of Advance Research, Ideas and Innovations in Technology, 6(4) www.IJARIIT.com.

MLA
Dr. Muthu Venkat T.. "Clinico haematological profile in beta Thalassemia trait." International Journal of Advance Research, Ideas and Innovations in Technology 6.4 (2020). www.IJARIIT.com.

Abstract

Introduction with Objectives: - The most common causes of microcytic hypochromic anemia in India are β Thalassemia & Iron deficiency anemia (IDA). β- Thalassemia is one of the most common single gene disorder in India with an overall prevalence of 3-4 %. Effective screening of β Thalassemia trait (βTT), decrease its incidence. Hb electrophoresis, serum iron profile & RBC indices are used to differentiate βTT from IDA .HbA2, red cell indices are observed as effective screening tests in βTT. Our main objective is to study the hemoglobin electrophoresis & RBC indices in β-Thalassemia Trait & to differentiate from IDA. Methodology: - A retrospective study of 50 patients (OP & IP) admitted in our KMC & hospital, Manipal was done. Study period was 1year (Aug 2015-Sep 2016). HbA2 level is studied by capillary zone electrophoresis method. Hb & red cell indices (MCV, MCH, RDW) were calculated. From red cell indices formulas –Mentzer (MI-cut off-13), Srivastava (SI-3.3), Shine & Lal(S&L-595), England & Frazer (E&F-1.39) were derived & serum iron profiles (Fe 2+, TIBC, Ferritin) was done to distinguish βTT from IDA. Vitamin B12 & folate assay was done . Sensitivity, specificity & Youden index were also calculated . Results:-All 50 pts were diagnosed as β-Thalassemia Trait (HbA2≥3.5 .Hb≤8gms/dl, MCV<60fL, MCH5x106/mm3, RDW>16%).Mean age group is 50yrs, M>F.3/50 cases (6%) shows ↑ HbF along with ↑HbA2.2/50 (4%) has ↑HbS .1 case shows IDA features with βTT. 49/50 (98%) cases of βTT showed-≤ cut off values of all index (MI,SI, S&LI ,E&FI). Serum iron profile was normal in 24/50 (48%), ↑ serum ferritin in 5/50 (10%).↑ vitamin B12 & folate levels seen in 9/50(18%).1 case showed ↓iron profile & ↑index. The MI is the most sensitive (>90%) & specific (>83%). Conclusion:-βTT & IDA are the M.C. causes of microcytic anemia. Hemogram parameters and RBC indices have significant role in βTT. HbA2, MCV & MI are the most sensitive tool to detect βTT.
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