The genomic DNA of seventeen silkworms were amplified with OPA-02 primer revealed 45 RAPD bands out of which 90% is polymorphism and 10% is monomorphic silkworm is polygenic. Silkworm variety 9 was not shown any amplification of bands however the varieties 1 and 2 showed one band each Some of the DNA fragments were strain specific and some could differentiate the multivoltine from the bivoltine strains or vice versa.Silkworm genetic resources that are being maintained in Hosur, Tamil Nadu, India, are yet to be adequately tapped to develop elite varieties that are subjected to a different agro-eco-climatic condition of the country like India. Molecular markers are known to provide an unambiguous estimate of the genetic variability of silkworm populations. Since they are independent confounding effects of environment, the genetic similarity among the seventeen silkworm varieties/strains and their genetic diversity and relationships were discussed. The amplification bands were very poor.