The use of plants in medicines has been prevalent since ancient times. Recent advances in Biotechnology have helped in the validation of their potential as drugs for the treatment of numerous diseases. Various parts of the Carica papaya, popularly grown for their fruits have also been used as ethnomedicine for a number of ailments like diabetes, indigestion, viral infections, and menstrual pain. The present study focusses on the preparation of extracts from the leaves of Male, Female and Hermaphrodite Carica papaya plants using solvents of varying polarities. The solvent was removed from the extract using a Rotary Vacuum Evaporator. A comparative study of the HPTLC analysis of these extracts revealed that the Leaf Extracts of Hermaphrodite Carica papaya plants had the maximum number of compounds present in them. In vitro cytotoxicity studies of the shortlisted extracts done using MTT assay on BHK-21 cells. The leaf extracts of Hermaphrodite Carica papaya plants showed no sign of toxicity up to a concentration of 100 µg/ ml, hence proves them to be safe for therapeutic use.