This paper is published in Volume-5, Issue-6, 2019
Area
Analytical Chemistry
Author
Dr. K. Ayyavoo
Co-authors
Dr. C. Tamilselvan
Org/Univ
Bioscience Research Foundation, Chennai, Tamil Nadu, India
Pub. Date
30 December, 2019
Paper ID
V5I6-1294
Publisher
Keywords
Acetamiprid and Bifenthrin, RP-HPLC, SANCO 3030/99 Rev.4, ICH Guideline

Citationsacebook

IEEE
Dr. K. Ayyavoo, Dr. C. Tamilselvan. Analytical method validation of acetamiprid+ bifenthrin formulation by Reverse Phase High Performance Liquid Chromatography (R-HPLC), International Journal of Advance Research, Ideas and Innovations in Technology, www.IJARIIT.com.

APA
Dr. K. Ayyavoo, Dr. C. Tamilselvan (2019). Analytical method validation of acetamiprid+ bifenthrin formulation by Reverse Phase High Performance Liquid Chromatography (R-HPLC). International Journal of Advance Research, Ideas and Innovations in Technology, 5(6) www.IJARIIT.com.

MLA
Dr. K. Ayyavoo, Dr. C. Tamilselvan. "Analytical method validation of acetamiprid+ bifenthrin formulation by Reverse Phase High Performance Liquid Chromatography (R-HPLC)." International Journal of Advance Research, Ideas and Innovations in Technology 5.6 (2019). www.IJARIIT.com.

Abstract

Many types of combinations of pesticides are being used effectively as insecticide, weedicide, growth regulators and may other applications successfully. To control termites and insecticide these combinations of acetamiprid and bifenthrin are being used effectively in the plant kingdom. Acetamiprid is an organic compound basically and the bifenthrin is and pyrethroid molecule. These molecules are being used as an insecticide; even though chemically different in nature. These molecules in different concentrations and combinations are being used over 100 countries; it is very important to detect and quantify with lesser time and cost-effective. This HPLC method is a simple method to quantify these two molecules within 17 minutes. The simple HPLC analytical method is a reversed-phase chromatography principle by using acetonitrile and 1% Ortho Phosphoric Acid (OPA) in the HPLC grade water 80:20 ratio. In this method Shimadzu C18 column is used with the dimension as 250mm length; 4.6 mm dimension and 5μm silica particle size packed. The detection was carried out at 210nm, with the Shimadzu detector supported with LC solution software. In this method, these acetamiprid was eluted at 3.6 minutes and the bifenthrin was eluted at 2.7 minutes. This analytical method was complying with all the validation parameters of SANCO 3029; version 4 and ICH guidelines.
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